Monday, 16 September 2019

Confocal Raman Microscope

Confocal Raman Microscope

Technical and Characterization Specifications:
  • Research grade optical microscope – Olumpus BX, complete microscope with 2 position motorized white light illuminator Koehler illumination by reflection (LED eqv 100 W)/ transmission (Halogen 30 W) with lamp housings, Abbe condenser and 2 objectives
    • 20X (NA=0.4 WD=1.3mm) , 100(NA=0.9 WD=0.21mm)
    • 50X LWD visible objective (NA=0.50 WD=10.6mm)
  • Raman spectrometer-
    Integrated imaging spectrometer with 4 gratings mounted on motorized turret with gratings: 600gr, 1200gr, 1800gr and 2400gr HORIBA Scientific CCD detector, TE air cooled (-60 ⁰ C), 1024x256 pixel, 16 bit
    Range: 200-1050 nm, QE: ~ 60% @ 700nm@ RT
  • Laser-
    • Air cooled solid state laser, 532 nm/ 25mW. Edge and Band pass filters set at 532 nm for measurements from 60 cm-1      
    • Air cooled solid state laser, 638 nm/30 mW. Edge and Band pass filters set at 638 nm for measurements from 50 cm-1
    • Air cooled solid state laser, 785 nm/100 mW. Edge and Band pass filters set at 785 nm for measurements from 50 cm-1
  • Spectral resolution – With 532 nm wavelength ≤ 1.4 cm-1/ pixel with 2400 grooves/mm
  • Confocal resolution: ≤ 500 nm lateral XY (with 532 nm)
  • Motorized PC controlled 6 position ND filter wheel for laser power adjustment (0.1%, 1%, 10%, 25%, 50%, 100%)
  • Motorized PC controlled confocal pinhole
  • Motorized PC controlled entrance slit for resolution selection Confocal coupling optics and
  • motorized filter selection
  • XY motorized stage, X=75 mm-Y=50mm.PC controlled by LabSpec software includes Z profiling
    XY specifications: minimum step size=10nm; repeatability=1µm; resolution: 100nm
  • LabSpec6 spectral software suite for the easy acquisition and analysis and analysis of Raman data. Includes control of the hardware and acquisition parameters, AUTO calibration, customizable methods, FLAT fluorescence subtraction, peak label and fit, image capture, smoothing, spectral subtraction etc.
    Including positioning joystick and an external controller
    SWIFT fast confocal Raman mapping
  •  Temperature controlled stage with a Platinum resistor temperature sensor. The stage is capable of heating / cooling both solid and liquid samples and supported by LNP95 liquid nitrogen pump.
    • Maximum temperature = 600⁰ C
    • Minimum temperature = - 196⁰ C with LNP95
    • Heating rate = 150⁰ C/min
    • Objective lens WD = 4.5mm
    • Aperture hole =2mm
    • Temperature accuracy =0.05⁰C