Wednesday, 23 January 2019

Circular Dichroism Spectropolarimeter

Circular Dichroism Spectropolarimeter

Technical and Characterization Specifications:
  • Determines the optical purity of chiral molecules
  • Measures the left-and right-handed circularly polarized light of optically active molecules.
  • Measures the chiroptical properties of solutions (chiral solutes, viz., peptides and proteins, dissolved in solvent)
  • Determines the chiroptical behaviour of chiral liquid crystal samples (as a function of temperature)
  • Determines the binding constants
  • Useful in determining kinetics of folding and unfolding of macromolecules
  • Provides thermodynamic parameters from temperature dependencies of CD signals
  • Equipped with Mettler FP90 hot stage
  • Comprises a variable wavelength polarimeter and absorption spectrophotometer (163-900 nm)
  • Up to four channels can be acquired simultaneously; the internal signals are selected from CD, FDCD, ORD, LD UV and Fluorescence
  • The acquired data are controlled by Jasco’s spectra manager
  • Light source: 150 W air cooled Xe lamp or 450 W water cooled Xe lamp (option)
  • Detector: Head-on photomultiplier tube
  • Modulator: Piezoelastic modulator
  • Spectral bandwidth: 0.01 to 15 nm; slit width: 1 to 3000 µm
  • Response: 0.5 msec to 32 sec
  • Scanning system: Continuous scan& step scan
  • Scanning speeds: 1 to 10000 nm/min (continuous scan)
  • Data interval: 0.025 to 10 nm (continuous scan); 0.1 to 100 nm (step scan); 0.5 msec to 60 min (time change)
  • CD full scale: ±10, 200, 2000 mdeg